Microbiome and Human Cancers
Summary of Research:
There are increasing research reports which establish the link between human microbiota and carcinogenesis. Pathogenic bacteria and altered microbiota interact with mucosal surface and as a result, epithelial damage can be seen especially in chronic infection conditions. It can be reckoned that gut microbiota-derived metabolites play key role in epithelial damage and carcinogenesis. More and more intriguing data are hinting at a much broader link between microbiota, bacterial infections, inflammation and carcinogenesis. The mechanism of this relationship between bacteria and human cancer is not well studied and understood, hence our laboratory aim to delineate this complex interaction among microbiota, bacterial infections, inflammation and carcinogenesis. We also plan to explore the role of bacteria, microbiota and their metabolites in enhancing cancer immunotherapy.
Towards understanding the link between intestinal microbiota and colorectal cancer
The gut microbiota is considered as a forgotten organ in human health and disease. It maintains gut homeostasis by various complex mechanisms. The dysbiosis or disruption of gut flora has been confirmed to be related to gastrointestinal diseases such as CRC and other diseases in different organs. Colorectal cancer is a multi-factorial and multi-stage involved disorder. The role for microorganisms that initiate and facilitate the process of colorectal cancer has become clear. Our group is studying the link of gut microbes in colorectal cancer causation. Clearly, more information is needed about mechanisms through which the microbial community promotes carcinogenesis. Understanding the events leading to development of intestinal carcinogenesis through gut microbes will provide novel insight into this pathology, and potentially lead to new therapeutic modalities targeting the microbiota.
Sensor/biosensor for detection of gut bacteria and their metabolites
The microbiota-derived metabolites such as Trimethylamine N-oxide (TMAO) have been implicated in human health and disease, and their early detection in the body fluids is thought to be significant in understanding pathogenesis and treatment of colorectal cancer and other diseases like Alzheimer’s disease, cardiovascular, cerebrovascular etc. Recent studies have identified a number of metabolites and microbes that have important roles in human health and disease and can be considered as biomarkers for diagnosis of different diseases.
Nishu Dalal, Dr. Payal Gulati, Anuradha Gupta, Manish Kushwaha, Govind Prakash
• CSIR Technology Award, 2012.
• Merck Millipore India Innovation Award, 2012.
• 'Young Scientist Award’ from IUBMB, 2006.
• ‘Young Investigator Award’ from HUPO, 2004.
• Merit Scholarship from DBT during M.Sc. at IIT Roorkee.
• US6896806 • US7018510 • US7022511 • US7179633 • US7267772
• US7429478 • US7736879 • US7906008 • US8080403 • US7396671
• EP1613739 • EP1611282 • EP1836133 • EP1831682 • EP1996520
• GB1613739 • GB1831682 • GB1836133 • GB1996520 • IN221931
• IN224060 • IN231224 • IN243806 • IN244344 • IN244801
• FI120771 • A2008/07816 • ZA 2005/7593 • CN352084 • SE529680
• CN 368495 • FI1613739 • SE1613739 • DE1613739 • CN200380110308.1
• FR1996520 • ZA 2007/5292 • ZA2007/05230 • ZA2007/05225 • ZA2005/07609
• FI1611282 • AU2004326112 • FR1611282 • SE1611282 • CNZL200480044738.2
• DK1836133 • AU2004326074 • AU2004326080 • DE1611282 • AU2004326113
• DK1831682 • AU2007226332 • BE1996520 • DE1996520 • CNZL200480044739.7
• CH1996520 • IT1996520 • ES1996520 • ZA2005/7592
- Jalandra R, Sharma M, Makharia GK, Kumar A* (2023) Inflammatory and deleterious role of gut microbiota-derived trimethylamine on colon cells. Front. Immunol. Vol. 13 - 2022 https://doi.org/10.3389/fimmu.2022.1101429 (*Corresponding Author)
- Jalandra R, Lakshmi GBVS, Dhiman TK, Sharma M, Kumar A* and Solanki PR* (2023) MIP based sensor for detection of gut microbiota derived Trimethylamine. J. Electrochem. Soc. 170 027504. DOI 10.1149/1945-7111/acb7a3 (*Corresponding Author)
- Dalal N, Dhiman TK, Lakshmi GBVS, Singh AK, Solanki PR and Kumar A* (2022) MIP based sensor for detection of gut microbiota-derived Indoxyl Sulfate using PANI-Graphene-NiS2. Mater. Today Chem. 26, 2022,101157. https://doi.org/10.1016/j.mtchem.2022.10115 (*Corresponding Author)
- Yadav AK, Verma D, Dalal N, Kumar A, Solanki PR (2022) Molecularly imprinted polymer-based nanodiagnostics for clinically pertinent bacteria and virus detection for future pandemics. Biosens. Bioelectron.: X, 12, 2022, 100257. https://doi.org/10.1016/j.biosx.2022.100257
- Tabassum Z, Mohan A, Mamidi N, Khosla A, Kumar A, Solanki PR, Malik and Girdhar M (2023) Recent trends in nanocomposite packaging films utilizing waste generated biopolymers: Industrial symbiosis and its implication in sustainability. IET Nanobiotechnol. DOI: 10.1049/nbt2.12122 (Accepted)
- Verma D, Sajwan RK, Lakshmi GBVS, Kumar A* and Solanki P* (2022) A Molecularly imprinted polymer based on novel polyaniline–zinc sulfide nanocomposite for electrochemical detection of trimethylamine n-oxide. Environ. Sci.Nano. 2022,9, 3992-4006. https://doi.org/10.1039/D2EN00624C (*Corresponding Author)
- Sharma R, Lakshmi GBVS, Kumar A* and Solanki P* (2022) Plypyrrole based molecularly imprinted polymer platform for Klebsiella pneumonia detection. ECS Sens. Plus 1 010603, http://dx.doi.org/10.1149/2754-2726/ac612c (*Corresponding Author)
- Verma D, Yadav AK, Chaudhary N, Mukherjee MD, Kumar P, Kumar A*, Solanki PR* (2022) Recent advances in understanding SARS-CoV-2 infection and updates on potential diagnostic and therapeutics for COVID-19. Coronaviruses. Vol.3, Issue 4, 2022. p 14-31.DOI: 10.2174/2666796703666220302143102 (*Corresponding Author)
- Mohan A, Girdhar M, Kumar R, Chaturvedi HS, Vadhel A, Solanki P, Kumar A, Kumar D, Mamidi N (2021) Polyhydroxy butyrate-based nanocomposites for bone tissue engineering. Pharmaceuticals 2021, 14, 1163. https://doi.org/10.3390/ph14111163
- Lakshmi GBVS, Yadav AK, Mehlawat N, Jalandra R, Solanki PR and Kumar A* (2021) Gut microbiota derived trimethylamine N-oxide (TMAO) detection through molecularly imprinted polymer based sensor. Sci Rep. 11, 1338 (2021). https://doi.org/10.1038/s41598-020-80122-6. (*Corresponding Author)
- Jalandra R, Dalal N, Yadav AK, Verma D, Sharma M, Singh R, Khosla A, Kumar A, Solanki PR(2021) Emerging role of trimethylamine-N-oxide (TMAO) in colorectal cancer. Appl Microbiol Biotechnol.Oct;105(20):7651-7660. doi:10.1007/s00253-021-11582-7.
- Dhiman TK, Lakshmi GBVS, Dave K, Roychoudhury A, Dalal N, Jha SK, Kumar A, Han KH and Solanki PR (2021) Rapid and label-free electrochemical detection of fumonisin-b1 using microfluidic biosensing platform based on Ag-CeO2 nanocomposite. J. Electrochem. Soc. DOI:10.1149/1945-7111/ac13cf
- Dalal N, Jalandra R, Bayal N, Yadav AK, Harshulika, Sharma M, Makharia GK, Kumar P, Singh R, Solanki P, Kumar A* (2021) Gut microbiota-derived metabolites in CRC progression and causation. J. Cancer Res. Clin. Oncol. DOI:10.1007/s00432-021-03729-w (*Corresponding Author)
- Yadav AK, Verma D, Chaudhary N, Kumar A and Solanki PR (2021) Aptamer based switches: A futuristic approach for Helicobacter pylori detection. Materials Letters 2022, 308 , 131239. https://doi.org/10.1016/j.matlet.2021.131239
- Hashmi Z, Dhiman TK, Chaudhary N, Singh AK, Kumar A* and Solanki PR (2021) L-Cysteine capped MgS quantum dots and their application in optical detection of levofloxacin. Front. Nanotechnol. 3, 2. DOI: 10.3389/fnano.2021.616186 (*Corresponding Author)
- Yadav AK, Verma D, Kumar P, Kumar A and Solanki PR (2021) The perspectives of biomarkers based electrochemical immunosensors, artificial intelligence and the internet of medical things towards COVID-19 diagnosis and management. Mater. Today Chem. Vol 20, 100443. https://doi.org/10.1016/j.mtchem.2021.100443
- Dalal N, Jalandra R, Sharma M, Prakash H, Makharia GK, Solanki P, Singh R, Kumar A* (2020) OMICS technologies for improved diagnosis and treatment of colorectal cancer: Technical advancement and major perspectives. Biomed Pharmacother. 131, 2020, 110648. https://doi.org/10.1016/j.biopha.2020.110648. (*Corresponding Author)
- Jalandra R, Yadav AK, Verma D, Dalal N, Sharma M, Singh R, Kumar A*, Solanki P (2020) Strategies and perspectives to develop SARS-COV-2 detection methods and diagnostics. Biomed Pharmacother. 129, 2020, 110446. https://doi.org/10.1016/j.biopha.2020.110446 (*Corresponding Author)
- Toor D, Wsson MK, Kumar P, Karthikeyan G, Kaushik NK, Goel C, Singh S, Kumar A and Prakash H (2019) Dysbiosis disrupts gut immune homeostasis and promotes gastric diseases. Int. J. Mol. Sci. 2019, 20, 2432.
- Gahoi S, Mandal RS, Ivanisenko N, Shrivastava P, Jain S, Singh AK, Raghunandanan MV, Kanchan S, Taneja B, Mandal C, Ivanisenko VA, Kumar A, Kumar R. Open Source Drug Discovery Consortium, Ramachandran S (2013) Computational Screening for New Inhibitors of M. tuberculosis Mycolyltransferases Antigen 85 Group of Proteins as Potential Drug Targets. J Biomol Struct Dynam 31(1):30-43
- Dhall P, Siddiqi TO, Ahmad A, Kumar R, Kumar A* (2012) Rapid monitoring and assessment of pollutional load in Dairy Waste Water. Anal. Methods, 2013,5, 977-981.(*Corresponding Author)
- Majhi MC, Behera AK, Kulshrestha NM, Mahmooduzafar, Kumar R, Kumar A* (2012) Extreme: A unified web repository of ex\tremophilic microorganisms. PLoS ONE, 8(5): e63083. (*Corresponding Author)
- Dhall P, Kumar R, Siddiqi TO and Kumar A* (2012) Selection of an apt support for immobilization of microbes for developing BOD biosensor. Anal. Methods 2013, 5, 1533-1541. (*Corresponding Author)
- Dhall P, Kumar R, Kumar A* (2012) BODBEADS – A ready to use seeding material for estimation of organic load of wastewater. Anal. Methods, 2012,4, 4101-4106 (*Corresponding Author)
- Kumar A*, Dhall P, Kumar R, (2012) Biological AOX removal of pulp mill plant effluent by Pseudomonas Aeruginosa – Bench study J Environ Eng Landsc Manag 21(4), 296-304. (*Corresponding Author)
- Gupta S, Saxena M, Saini N, Mahmooduzzafar, Kumar R, Kumar A* (2012) An Effective Strategy for a Whole-Cell Biosensor Based on Putative Effector Interaction Site of the Regulatory DmpR Protein. PLoS ONE 7(8): e43527 (*Corresponding Author)
- Saini R, Kapoor R, Kumar R, Siddiqui TO and Kumar A (2011) CO2 utilizing microbes-A comprehensive review. Biotechnol Adv 29(6):949-60.
- Suresh PS, Kumar R and Kumar A (2011) Three dimensional model for N-terminal A domain of DmpR (2-dimethylphenol) protein based on secondary structure prediction and fold recognition. In Silico Biol 10(5):223-33
- Singh Y, Dhall P, Mathur R, Jain R, Thakur V, Kumar V, Kumar R and Kumar A (2011) Bioremediation of pulp and paper mill effluent by tannic acid degrading Enterobacter sp. Water Air Soil Pollut 218:693-701
- Rani A, Jain S, Dureja P, Kumar R and Kumar A (2011) Synthesis of some 3-(5-chloro-1,3-diaryl-1H-pyrazol-4-yl)-1-arylprop-2-en-1-ones and 1,5-bis(5-chloro-1,3-diaryl-1H-pyrazol-4-yl)pent-1,4-diene-3-ones and their antimicrobial activity. Indian J Chem B Org 50B:1534-1531
- Joshi S, Bisht GS, Rawat DS, Kumar A, Kumar R, Maiti S and Pasha S (2010) Interaction studies of novel cell selective antimicrobial peptides with model membranes and E. coli ATCC 11775. Biochim Biophys Acta 1798(10):1864-75
- Kumar A, Dhall P and Kumar R (2010) Redefining BOD:COD ratio of pulp mill industrial wastewaters in BOD analysis by formulating a specific microbial seed. Int Biodeterior Biodegradation 64(3):197-202
- Kulshreshtha NM, Kumar A, Dhall P, Bisht G, Pasha S, Gupta S and Kumar R (2010) Neutralization of alkaline industrial wastewaters using Exiguobacterium sp. Int Biodeterior Biodegradation 64(3):191-196
- Tiku DK, Kumar A, Chaturvedi R, Makhijani SD, Manoharan A and Kumar R (2010) Holistic bioremediation of pulp mill effluents using autochthonous bacteria. Int Biodeterior Biodegradation 64(3): 173-183
- Saini R, Majhi MC, Kapoor R, Kumar A and Kumar R (2009) CSD: A database of CO2 utilizing strains. Environ Model Software 24(9):1133-1134
- Suresh PS, Kumar A, Kumar R and Singh VP (2008) An Insilco approach to bioremediation: Laccase as a case study. J Mol Graph Model 26(5):845-849
- Dhall P, Kumar A, Joshi A, Saxsena TK, Manoharan A, Makhijani SD, Singh VP and Kumar R (2008) Quick and reliable estimation of BOD load of beverage industrial wastewater by developing BOD biosensor. Sensor Actuator B Chem 133(2):478-483
- Bisht GS, Rawat DS, Kumar A, Kumar R and Pasha S (2007) Antimicrobial activity of rationally designed amino terminal modified peptides. Bioorg Med Chem Lett 17(15):4343-4346
- Tiku DK, Kumar A, Sawhney S, Singh VP and Kumar R (2007) Effectiveness of treatment technologies for wastewater pollution generated by Indian pulp mills. Environ Monit Assess 132:453-466
- Rastogi S, Kumar A, Mehra NK, Makhijani SD, Manoharan A, Gangal V, Kumar R (2003) Development and characterization of a novel immobilized microbial membrane for rapid determination of biochemical oxygen demand load in industrial waste-waters. BiosensBioelectron 18 (1):23-29