Dual-wavelength X-ray generator integrated with image plates and cryogenic systems
The current X-ray generator setup in NII consists of SuperBright rotating anodes (Copper and Chromium) that produce high flux X-rays with two wavelengths (CuKα, 1.54 Å and CrKα, 2.29 Å). Integrated with the generator are two R-AXIS IV++ image plates. The copper beam enables X-ray data collection from biological macromolecule crystals up to as high as 1.5 Å resolution; whereas, the chromium beam allows to carry out ab initio structure solution using single anomalous dispersion phasing from the sulphur anomalous signal. Data at low temperature (typically 100 K) can also be collected using cryogenic system. Such an integrated X-ray crystallography infrastructure in NII has been extremely useful, for NII scientists, in determining novel protein structures particularly from pathogenic organisms and moreover aids studying protein/ligand and protein/drug interactions. A skilled technical person oversees the maintenance and operation of the system and helps users in crystal mounting, data collection and data processing. Over the years, scientists from various Indian institutes also have been collecting X-ray diffraction data at NII.
For using the above infrastructure please 👉 CLICK HERE to download the form