Skip to main content

Orbitrap Exploris 240 (LC-MS)

Orbitrap Exploris 240 (LC-MS)

Mass Spectrometer for Proteomics
The Centralized Mass Spectrometry Facility (CMSF) of NII provides support and services for Protein Identification and Characterization.
"
Thermo Orbitrap Exploris 240: The Thermo Orbitrap Exploris 240 HR-MS with Nano-LC has been recently installed in April 2023. As the name suggests, the Exploris 240 system has the resolving power (FWHM) in the range 15,000 to 240,000 at m/z 200 with exceptional mass accuracy. It enables experimental versatility without compromising data certainty. The MS performance has been strongly backed up with Vanquish Neo-UHPLC (Nano-LC) system that has been installed for the very first time in North India. It has the capacity to deliver flow rates in the range 1 nLmin-1 to 100 uLmin-1 to exhibit a broad range of high-sensitivity LC-MS workflows.
olumn specifically designed for Neo-LC delivers best-in-class separation of proteome. Recently launched double nanoViper (DNV) PepMap Neo columns with the Vanquish Neo UHPLC system allows run at up to 1500 bar. Neo-LC can deliver high performance for proteomics experiments consisting of sample amounts ranging from as low as 10 ng to higher load of 200 ng for both peptide and protein identification. The system has high efficacy designed for scientists in proteomics domain that yields faster turnaround time for sample-to-results. Additionally, easy-to-use interface of Xcalibur software provides efficient data acquisition, data processing, data analysis and output generation. MS data analysis softwares i.e. Proteome Discoverer 3.0 (soon to be upgraded to ver. 3.1) & Mascot Server 2.4 provides high-throughput qualitative and quantitative proteomics, identification of intact proteins, peptides, MS/MS analysis and especially the identification of post-translational modifications.

For using the above infrastructure please 👉 CLICK HERE to submit the form
👉 CLICK HERE for SOPs for In-Solution digestion form
👉 CLICK HERE for SOPs for In-Gel digestion
👉 CLICK HERE for SOPs for Desalting procedure