Skip to main content

Mass - Spectrometer

Mass - Spectrometer

Mass Spectrometer 

The Centralized Mass Spectrometry Facility (CMSF) of NII provides support and services for Protein Identification and Characterization. Core activities includes identification of proteins and determination of post-translational modifications using state of art mass spectrometry. Following are the major equipments at the CMSF:
Liquid Chromatography-Mass Spectrometry (LC-MS/MS) (Make: Thermo Scientific, USA)
A) Mass Spectrometer: Orbitrap Exploris 240
i) UHPLC: Vanquish Neo
ii) MS Data Analysis Software: Proteome Discoverer 3.0 & Mascot Server 2.4

Thermo Orbitrap Exploris 240: The Thermo Orbitrap Exploris 240 HR-MS with Nano-LC has been recently installed in April 2023. As the name suggests, the Exploris 240 system has the resolving power (FWHM) in the range 15,000 to 240,000 at m/z 200 with exceptional mass accuracy. It enables experimental versatility without compromising data certainty. The MS performance has been strongly backed up with Vanquish Neo-UHPLC (Nano-LC) system that has been installed for the very first time in North India. It has the capacity to deliver flow rates in the range 1 nLmin-1 to 100uLmin-1 to exhibit a broad range of high-sensitivity LC-MS workflows.
👉 For more details CLICK HERE
👉 CLICK HERE to submit the form
👉 CLICK HERE for SOPs for In-Solution digestion form
👉 CLICK HERE for SOPs for In-Gel digestion
👉 CLICK HERE for SOPs for Desalting procedure

Gas Chromatography - Mass Spectrometer (GC-MS): The GC-MS (Agilent 7890A) coupled with 5975C MSD with Triple-Axis Detector (TAD) is a high-performance system. Equipped with advanced separation capabilities and powerful new productivity features; it also has real-time self-monitoring instrument intelligence powered by a 5th-generation electronic pneumatics control (EPC). Additionally, digital electronics for retention time locking (RTL) precision delivers exceptional performance and reliability. Faster oven cool-down and robust backflush capability coupled with advanced automation features and faster GC/MS oven ramps results in enhanced productivity.
👉 For More Details CLICK HERE
👉 CLICK HERE to submit the form