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अनीशकुमार ए.जी.

Summary of Research:

Nutrients, metabolism and gene expression
A healthy life depends on the balance between nutrient uptake and the metabolism of the individual. While macronutrients serve as the source of both energy and structural building blocks of the organism, micronutrients are essential for various activities that are essential for metabolic processes including energy generation. The energy and chemical homeostasis of the body is maintained by multiple pathways and the ratio of different macronutrients in the staple diet determines which pathway is predominant in an organism at a given time. The bioavailability of the micronutrients can also differentially influence these pathways, setting stage for a complex interaction network of different nutrients and pathways, the outcome of which will determine the health of the individual. Such interactions could also be influenced by the genetic polymorphisms, possibly creating individualistic differences in the utilization and requirements of macro and micro nutrients. A detailed understanding of these interactions is essential to devise next-generation public health policy regarding nutritional requirements. 

Since metabolic homeostasis involves multiple organs, an imbalance in one system affects others leading to a domino effect on multiple organs. To elucidate the effects of nutrient-metabolic interactions, a systems level approach that integrates multiple organ systems, which takes into account differential molecular changes in organ systems is required. Moreover, unraveling the cause-effect relationship between the organ specific molecular functions and the overall physiological output is essential to develop technologies and pharmaceuticals to advance public health. 

We use genomics and molecular biology tools to elucidate the effect of nutrient-metabolic interactions in multiple organ systems including skeletal muscle, liver, intestine bones, and immune system using vitamin D metabolism as a model system. The following broad areas are being addressed in the lab

Maintenance of metabolic homeostasis by vitamin D

Metabolic regulation of gene expression in immune cells

Regulation of protein synthesis in Mycobacterium tuberculosis
Apart from the nutrient-metabolism interactions in mammals, we also address how gene expression is regulated in human pathogen Mycobacterium tuberculosis. Though approximately 25% of the human population is expected to be infected with Mycobacterium tuberculosis, only 10% of them, based on their immune and metabolic status manifest the pathology. While modulation of protein synthesis has been implicated in pathogenicity, the mechanisms are unknown. We address various mechanisms by which M. tb protein synthesis is regulated to cope with host induced stress conditions.

Group Members:
Mr. Khem Singh Negi, Ms. Anamica Das, Mr. Gagan Dev, Ms. Neha, Mr. Chen Chongtham, Mr. Somdeb Chattopadhyay
  • Soman S, Chattopadhyay S, Ram S, Nandicoori VK, Arimbasseri GA. (2023) Codon optimality has minimal effect on determining translation efficiency in mycobacterium tuberculosis. Sci Rep. 13(1):415. doi: 10.1038/s41598-022-27164-0. PMID: 36624167
  • Dev G, Chawla AS, Gupta S, Bal V, George A, Rath S, Arimbasseri GA. (2022) Differential Regulation of Two Arms of mTORC1 Pathway Fine-Tunes Global Protein Synthesis in Resting B Lymphocytes. Int J Mol Sci. 23(24):16017. doi: 10.3390/ijms232416017.
  • Ratra Y, Kumar N, Saha MK, Bharadwaj C, Chongtham C, Bais SS, Medigeshi G, Arimbasseri GA, Basak S. (2022) A Vitamin D-RelB/NF-κB Pathway Limits Chandipura Virus Multiplication by Rewiring the Homeostatic State of Autoregulatory Type 1 IFN-IRF7 Signaling. J Immunol. 209 (3): 559–568 . doi: 10.4049/jimmunol.2101054.
  • Kumar S, Khan MZ, Khandelwal N, Chongtham C, Singha B, Dabla A, Behera D, Singh A, Gopal B, Arimbasseri GA, Kamat SS, Nandicoori VK. (2022) Mycobacterium tuberculosis Transcription Factor EmbR Regulates the Expression of Key Virulence Factors That Aid in Ex Vivo and In Vivo Survival. mBio. e0383621. doi: 10.1128/mbio.03836-21.
  • Das A, Gopinath SD, Arimbasseri GA. (2021) Systemic ablation of vitamin D receptor leads to skeletal muscle glycogen storage disorder in mice. J. Cachexia, Sarcopenia and Muscle. 13(1):467-480. DOI: 10.1002/jcsm.12841. PMID: 34877816
  • Pani T, Rajput K, Kar A, Sharma H, Basak R, Medatwal N, Saha S, Dev G, Kumar S, Gupta S, Mukhopadhyay A, Malakar D, Maiti TK, Arimbasseri AG, Deo SVS, Sharma RD, Bajaj A, Dasgupta U. (2021) Alternative splicing of ceramide synthase 2 alters levels of specific ceramides and modulates cancer cell proliferation and migration in Luminal B breast cancer subtype. Cell Death Dis. 12(2):171. doi: 10.1038/s41419-021-03436-x. PMID: 33568634
  • Mishra S, Sevak JK, Das A, Arimbasseri GA, Bhatnagar S, Gopinath SD. (2020) Umbilical cord tissue is a robust source for mesenchymal stem cells with enhanced myogenic differentiation potential compared to cord blood. Sci Rep. 10(1):18978. doi: 10.1038/s41598-020-75102-9. PMID: 33149204
  • Umar D, Das A, Gupta S, Chattopadhyay S, Sarkar D, Mirji G, Kalia J, Arimbasseri GA, Durdik JM, Rath S, George A, Bal V. (2020) Febrile temperature change modulates CD4 T cell differentiation via a TRPV channel-regulated Notch-dependent pathway. Proc Natl Acad Sci U S A. DOI: 10.1073/pnas.1922683117 (PMID: 32839313)
  • Chawla AS, Khalsa JK, Dhar A, Gupta S, Umar D, Arimbasseri GA, Bal V, George A, Rath S. (2020) A role for cell-autocrine interleukin-2 in regulatory T-cell homeostasis, Immunology, 160(3):295-309. doi: 10.1111/imm.13194 (PMID: 32187647)
  • Dhar A, Chawla M, Chattopadhyay S, Oswal N, Umar D, Gupta S, Bal V, Rath S, George A, Arimbasseri GA, Basak S. (2020) Role of NF-kappaB2-p100 in regulatory T cell homeostasis and activation. Sci Rep. 9(1):13867. doi: 10.1038/s41598-019-50454-z (PMID: 31554891)
  • Bhalla P, Shukla A, Vernekar DV, Arimbasseri AG, Sandhu KS, Bhargava P.  (2019) Yeast PAF1 complex counters the pol III accumulation and replication stress on the tRNA genes. Sci Rep., 9(1):12892. doi: 10.1038/s41598-019-49316-5
  • Khalsa JK, Chawla AS, Prabhu SB, Vats M, Dhar A, Dev G, Das N, Mukherjee S, Tanwar S, Banerjee H, Durdik JM, Bal V, George A, Rath S*, Arimbasseri GA*. Functionally significant metabolic differences between B and T lymphocyte lineages. Immunology. 158(2):104-120. doi: 10.1111/imm.13098 (PMID: 31318442) *co-corresponding author
  • Arimbasseri GA. (2018) Interactions between RNAP III transcription machinery and tRNA processing factors. Biochim Biophys Acta Gene Regul Mech.  1861(4):354-360. doi: 10.1016/j.bbagrm.2018.02.003 (PMID: 29428193)


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